1×, ph 8.0 te buffer Search Results


vwr extra pure  (Chem Impex International)
95
Chem Impex International vwr extra pure
Vwr Extra Pure, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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t0224  (Teknova)
93
Teknova t0224
T0224, supplied by Teknova, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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m tris hcl ph8 0  (Jena Bioscience)
92
Jena Bioscience m tris hcl ph8 0
M Tris Hcl Ph8 0, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chondroitinase abc solution  (AMS Biotechnology)
96
AMS Biotechnology chondroitinase abc solution
Chondroitinase Abc Solution, supplied by AMS Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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te buffer  (Teknova)
94
Teknova te buffer
Te Buffer, supplied by Teknova, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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te buffer - by Bioz Stars, 2026-03
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tris hcl  (Chem Impex International)
95
Chem Impex International tris hcl
Northern blot analyses of <t>intracellular</t> <t>aminoacylation</t> of tRNAPyl isoacceptors. Total RNA was obtained from E. coli cells grown in the presence or absence of 3-I-Phe (1 mM) expressing IFRS and (A) either one or five gene copies of tRNAPylACU or (B) one gene copy of tRNAPylCAG, from the pCAM plasmid. Total RNA was suspended in either 10 mM sodium acetate, pH 4.5 (acylated), or 200 mM <t>Tris,</t> pH 8.0 (deacylated, OH−). Positions of acylated and deacylated tRNAPyl isoacceptors are indicated.
Tris Hcl, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hepes  (Thermo Fisher)
94
Thermo Fisher hepes
Northern blot analyses of <t>intracellular</t> <t>aminoacylation</t> of tRNAPyl isoacceptors. Total RNA was obtained from E. coli cells grown in the presence or absence of 3-I-Phe (1 mM) expressing IFRS and (A) either one or five gene copies of tRNAPylACU or (B) one gene copy of tRNAPylCAG, from the pCAM plasmid. Total RNA was suspended in either 10 mM sodium acetate, pH 4.5 (acylated), or 200 mM <t>Tris,</t> pH 8.0 (deacylated, OH−). Positions of acylated and deacylated tRNAPyl isoacceptors are indicated.
Hepes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pellet tris low edta suspension buffer  (Teknova)
94
Teknova pellet tris low edta suspension buffer
Northern blot analyses of <t>intracellular</t> <t>aminoacylation</t> of tRNAPyl isoacceptors. Total RNA was obtained from E. coli cells grown in the presence or absence of 3-I-Phe (1 mM) expressing IFRS and (A) either one or five gene copies of tRNAPylACU or (B) one gene copy of tRNAPylCAG, from the pCAM plasmid. Total RNA was suspended in either 10 mM sodium acetate, pH 4.5 (acylated), or 200 mM <t>Tris,</t> pH 8.0 (deacylated, OH−). Positions of acylated and deacylated tRNAPyl isoacceptors are indicated.
Pellet Tris Low Edta Suspension Buffer, supplied by Teknova, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tris  (Chem Impex International)
95
Chem Impex International tris
Northern blot analyses of <t>intracellular</t> <t>aminoacylation</t> of tRNAPyl isoacceptors. Total RNA was obtained from E. coli cells grown in the presence or absence of 3-I-Phe (1 mM) expressing IFRS and (A) either one or five gene copies of tRNAPylACU or (B) one gene copy of tRNAPylCAG, from the pCAM plasmid. Total RNA was suspended in either 10 mM sodium acetate, pH 4.5 (acylated), or 200 mM <t>Tris,</t> pH 8.0 (deacylated, OH−). Positions of acylated and deacylated tRNAPyl isoacceptors are indicated.
Tris, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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isobutyl chloroformate  (Chem Impex International)
95
Chem Impex International isobutyl chloroformate
Northern blot analyses of <t>intracellular</t> <t>aminoacylation</t> of tRNAPyl isoacceptors. Total RNA was obtained from E. coli cells grown in the presence or absence of 3-I-Phe (1 mM) expressing IFRS and (A) either one or five gene copies of tRNAPylACU or (B) one gene copy of tRNAPylCAG, from the pCAM plasmid. Total RNA was suspended in either 10 mM sodium acetate, pH 4.5 (acylated), or 200 mM <t>Tris,</t> pH 8.0 (deacylated, OH−). Positions of acylated and deacylated tRNAPyl isoacceptors are indicated.
Isobutyl Chloroformate, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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j60618 ak  (Thermo Fisher)
94
Thermo Fisher j60618 ak
Northern blot analyses of <t>intracellular</t> <t>aminoacylation</t> of tRNAPyl isoacceptors. Total RNA was obtained from E. coli cells grown in the presence or absence of 3-I-Phe (1 mM) expressing IFRS and (A) either one or five gene copies of tRNAPylACU or (B) one gene copy of tRNAPylCAG, from the pCAM plasmid. Total RNA was suspended in either 10 mM sodium acetate, pH 4.5 (acylated), or 200 mM <t>Tris,</t> pH 8.0 (deacylated, OH−). Positions of acylated and deacylated tRNAPyl isoacceptors are indicated.
J60618 Ak, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mops buffer  (Thermo Fisher)
94
Thermo Fisher mops buffer
Northern blot analyses of <t>intracellular</t> <t>aminoacylation</t> of tRNAPyl isoacceptors. Total RNA was obtained from E. coli cells grown in the presence or absence of 3-I-Phe (1 mM) expressing IFRS and (A) either one or five gene copies of tRNAPylACU or (B) one gene copy of tRNAPylCAG, from the pCAM plasmid. Total RNA was suspended in either 10 mM sodium acetate, pH 4.5 (acylated), or 200 mM <t>Tris,</t> pH 8.0 (deacylated, OH−). Positions of acylated and deacylated tRNAPyl isoacceptors are indicated.
Mops Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Northern blot analyses of intracellular aminoacylation of tRNAPyl isoacceptors. Total RNA was obtained from E. coli cells grown in the presence or absence of 3-I-Phe (1 mM) expressing IFRS and (A) either one or five gene copies of tRNAPylACU or (B) one gene copy of tRNAPylCAG, from the pCAM plasmid. Total RNA was suspended in either 10 mM sodium acetate, pH 4.5 (acylated), or 200 mM Tris, pH 8.0 (deacylated, OH−). Positions of acylated and deacylated tRNAPyl isoacceptors are indicated.

Journal: ACS synthetic biology

Article Title: Efficient Reassignment of a Frequent Serine Codon in Wild-Type Escherichia coli

doi: 10.1021/acssynbio.5b00197

Figure Lengend Snippet: Northern blot analyses of intracellular aminoacylation of tRNAPyl isoacceptors. Total RNA was obtained from E. coli cells grown in the presence or absence of 3-I-Phe (1 mM) expressing IFRS and (A) either one or five gene copies of tRNAPylACU or (B) one gene copy of tRNAPylCAG, from the pCAM plasmid. Total RNA was suspended in either 10 mM sodium acetate, pH 4.5 (acylated), or 200 mM Tris, pH 8.0 (deacylated, OH−). Positions of acylated and deacylated tRNAPyl isoacceptors are indicated.

Article Snippet: Biochemical Characterization of the IFRS/tRNA Pyl Pair The aminoacylation assays were carried out for 60 min at 37 °C in aminoacylation buffer containing 100 mM Tris-HCl (pH 7.0), 10 mM MgCl 2 , 50 mM potassium chloride, and 5 mM DTT, with 1 mM 3-I-Phe (ChemImpex), 2 mM ATP, 500 nM IFRS, and 5 µM 32 P 3′-end-labeled transcript.

Techniques: Northern Blot, Expressing, Plasmid Preparation